616.234.5000

Molecular Processing

Pathology and Biorepository Core

The Pathology and Biorepository Core offers blood and tissue processing, nucleic acid extractions, sample quality assessment, large-scale aliquoting and more. Our highly trained staff will work with you to determine the best method for your exact experimental needs. In addition to our full-service offerings, we also have several pieces of shared equipment available for use. 

Service requests and pricing are managed through Agilent CrossLab (See Schedule Equipment and/or Request Services). Current CrossLab users can login here.

VARI staff that need a login (or further CrossLab support) should contact Lori Moon and MSU staff that need a login should contact Erica Lange. External users that need an CrossLab account/login can go here for account creation. After logging in, external users should click “list all cores” under the Core Facilities section in the left menu and scroll down to “Cores at Van Andel Institute.”

Questions? Please email pbc@vai.org.

Services

Nucleic acid purifications

  • DNA and RNA extractions from tissue, blood, cells, FFPE samples, etc.
  • Clean up and concentration of DNA or RNA samples
  • Measuring sample concentration and integrity

Blood and frozen tissue processing

  • Blood fractionation
  • Cell sorting
  • Homogenization and aliquoting of frozen tissue using Covaris cryoPREP pulverizer

Sample aliquoting

  • Routine aliquoting of fresh blood products (i.e., plasma, buffy coat, whole blood)
  • Frozen aliquoting of liquids or tissues using the CryoXtract CXT 353
Equipment available for shared use

Qiagen QIAcube
Automated purification of DNA, RNA, or protein for up to 12 samples

Miltenyi autoMACS Pro Separator
Automated cell sorting using antibody-labeled magnetic beads

Qiagen TissueLyser II
Disrupts biological samples through high-speed shaking with steel beads